Second division runners-up Scalby secured a 4-2 win against Ayton at the Flamingo Land Stadium.
Man of the match Rob Speight led the way with a hat-trick, Taylor Jordan also notching for an in-form Scalby side.
Tommy Day and Ian Laing, who had started the game in goal, netted for Ayton, whose man of the match was left-back Jaymaine White.
Falsgrave Athletic claimed a 3-2 win at home to FC Rosette.
A brace from Dane Robinson put Rosette 2-1 ahead at the interval, his first a chipped shot and the second a long-range strike.
Falsgrave rallied after the break, scoring twice to secure all three points.
The Rosette man of the match was Al Wray.
Goldsborough United eased to a 5-0 win at basement club Old Vic.